解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their potential activity in multiple sclerosis (MS), serum levels of KLK1, 6, 7, 8 and 1...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.085
更新日期:2008-06-01 00:00:00
abstract::Type I interferons (IFNs) produced primarily by plasmacytoid dendritic cells (pDCs) as part of the innate immune response to infectious agents induce the maturation of myeloid DCs and enhance antigen presentation. Type I IFNs also enhance apoptosis of virus-infected cells, stimulate cross priming and enhanced presenta...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/bc.2008.051
更新日期:2008-05-01 00:00:00
abstract::The lysosomal cysteine proteases cathepsin B and L play important roles in tumor cell invasion. An imbalance between these cathepsins and their endogenous inhibitors, the cystatins, has been associated with development of the metastatic phenotype. Accordingly, many studies have indicated potential use of cystatins in ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.035
更新日期:2008-04-01 00:00:00
abstract::Forkhead box O (FoxO) transcription factors are important downstream targets of the PI3K/Akt signaling pathway and crucial regulators of cell fate. This function of FoxOs relies on their ability to control diverse cellular functions, including proliferation, differentiation, apoptosis, DNA repair, defense against oxid...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2008.033
更新日期:2008-03-01 00:00:00
abstract::Abstract Few genes are known to be involved in renal cell carcinoma (RCC) development and progression. The cell-specific transcription factor hepatocyte nuclear factor 4 alpha (HNF4 alpha) is down-regulated in RCC and we have shown that HNF4 alpha inhibits cell proliferation in the embryonic kidney cell line HEK293. T...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.011
更新日期:2008-02-01 00:00:00
abstract::The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.010
更新日期:2008-01-01 00:00:00
abstract::We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human HaCaT keratinocytes as a surrogate cell line for NHK. HaCaT cells osmo-dependentl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.140
更新日期:2007-12-01 00:00:00
abstract::Cathepsin E (CE) is an intracellular aspartic proteinase implicated in various physiological and pathological processes, yet its actual roles in vivo remain elusive. To assess the physiological significance of CE expression in tumor cells, human CE was stably expressed in human prostate carcinoma ALVA101 cells express...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.154
更新日期:2007-11-01 00:00:00
abstract::In bacteria, UGA stop codons can be recoded to direct the incorporation of selenocysteine into proteins on the ribosome. Recoding requires a selenocysteine incorporation sequence (SECIS) downstream of the UGA codon, a specialized translation factor SelB, and the non-canonical Sec-tRNASec, which is formed from Ser-tRNA...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.108
更新日期:2007-10-01 00:00:00
abstract::Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.104
更新日期:2007-09-01 00:00:00
abstract::In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.083
更新日期:2007-08-01 00:00:00
abstract::The major enzymatic activity of the ribosome is the catalysis of peptide bond formation. The active site -- the peptidyl transferase center -- is composed of ribosomal RNA (rRNA), and interactions between rRNA and the reactants, peptidyl-tRNA and aminoacyl-tRNA, are crucial for the reaction to proceed rapidly and effi...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2007.077
更新日期:2007-07-01 00:00:00
abstract::TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.066
更新日期:2007-06-01 00:00:00
abstract::The N-terminal propeptide domains of several cathepsin L-like cysteine proteases have been shown to possess potent inhibitory activity. Here we report the first kinetic characterisation of the inhibition properties of the cathepsin V propeptide (CatV PP). Using a facile recombinant approach we demonstrate expression, ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.053
更新日期:2007-05-01 00:00:00
abstract::The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. Experiments using anti-peptide antisera indicated that this 556-aa protein is nuclear throughout the cell cycle and excluded from condensed chromatin during mitosis. Based on its electrophoretic migration and sensitivity to ph...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.045
更新日期:2007-04-01 00:00:00
abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.036
更新日期:2007-03-01 00:00:00
abstract::In this study, we tested the biological activity of a novel acylated form of (Pro3)glucose-dependent insulinotropic polypetide [(Pro3)GIP] prepared by conjugating palmitic acid to Lys16 to enhance its efficacy in vivo by promoting binding to albumin and extending its biological actions. Like the parent molecule (Pro3)...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.019
更新日期:2007-02-01 00:00:00
abstract::This is the first report describing the enhancement of xylanase activity by the neutral amino acid glycine. Xylanase activity is increased seven-fold at alkaline pH in the presence of glycine and its pH optimum is shifted from pH 7 to 8 without using any protein engineering techniques. Analysis of the steady-state kin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.007
更新日期:2007-01-01 00:00:00
abstract::It is now appreciated that mitochondria form tubular networks that adapt to the requirements of the cell by undergoing changes in their shape through fission and fusion. Proper mitochondrial distribution also appears to be required for ATP delivery and calcium regulation, and, in some cases, for cell development. Whil...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.193
更新日期:2006-12-01 00:00:00
abstract::The BCL2 family of genes (B-cell CLL/lymphoma 2; Bcl-2) plays a pivotal role in the highly regulated process of apoptosis. We have recently cloned a newly identified member of this family, BCL2L12, which was found to be differentially expressed in many tumors. It is known that topotecan and methotrexate act through in...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.203
更新日期:2006-12-01 00:00:00
abstract::Vascular injury triggers endothelial exocytosis of granules, releasing pro-inflammatory and pro-thrombotic mediators into the blood. Nitric oxide (NO) and reactive oxygen species (ROS) limit vascular inflammation and thrombosis by inhibiting endothelial exocytosis. NO decreases exocytosis by regulating the activity of...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.173
更新日期:2006-10-01 00:00:00
abstract::The molecular architectures of the catalytic core of protein phosphatase 1 (PP1) and protein phosphatase 2B (PP2B) are similar, and both contain a beta12-beta13 loop that consists of non-conserved residues. A truncation mutant containing the PP2B catalytic domain has previously been constructed in our laboratory, and ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.183
更新日期:2006-10-01 00:00:00
abstract::The ligand-activated aryl hydrocarbon receptor (AHR) is known to modulate many genes in a highly cell-specific manner, either directly or indirectly via secondary effects. In contrast, little is known about the effects of AHR deficiency on gene expression balance. We compared the transcriptome of CD4 T cells from AHR-...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.151
更新日期:2006-09-01 00:00:00
abstract::One of the control mechanisms of cathepsin B biosynthesis and trafficking operates through alternative splicing of pre-mRNA. An mRNA lacking exon 2 is more efficiently translated than that containing all exons, and may be responsible for elevated biosynthesis and enzyme routing to the extracellular space, with critica...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.125
更新日期:2006-08-01 00:00:00
abstract::The lysosomal lumen contains numerous acidic hydrolases involved in the degradation of carbohydrates, lipids, proteins, and nucleic acids, which are basic cell components that turn over continuously within the cell and/or are ingested from outside of the cell. Deficiency in almost any of these hydrolases causes accumu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.135
更新日期:2006-08-01 00:00:00
abstract::Few therapeutic options are offered to treat inflammation and alveolar wall destruction in emphysema. The effect of recombinant human pre-elafin, an elastase inhibitor, was evaluated in porcine pancreatic elastase (PPE)-induced emphysema in C57BL/6 mice. In a first protocol, mice received a single instillation of pre-...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.114
更新日期:2006-07-01 00:00:00
abstract::The kallikrein-related peptidase (KLK) gene family of 15 serine proteases encodes many proteins, including prostate specific antigen (PSA or KLK3), that are well described and/or are potential biomarkers for hormone-related cancers. Variant mRNA transcripts produced by alternative splicing, polyadenylation or AUG site...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.088
更新日期:2006-06-01 00:00:00
abstract::Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.098
更新日期:2006-06-01 00:00:00
abstract::There are enzymatic and non-enzymatic mechanisms that generate NO* from nitrite in blood, stomach, saliva, urine and skin. In blood vessels, nitrite-derived NO* can provide protection via compensatory vasodilation during hypoxia, and in various body fluids it may have antibacterial activity. In the skin, nitrite-deriv...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.065
更新日期:2006-05-01 00:00:00
abstract::The three isoforms of human Ins(1,4,5)P3 3-kinase (IP3K) show remarkable differences in their intracellular targeting. Whereas predominant targeting to the cytoskeleton and endoplasmic reticulum has been shown for IP3K-A and IP3K-B, rat IP3K-C shuttles actively between the nucleus and cytoplasm. In the present study w...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.075
更新日期:2006-05-01 00:00:00
abstract::The immunoproteasome subunit low molecular weight protein 2 (LMP2) codon 60 polymorphism has been associated with autoimmune diseases. It has also been demonstrated to influence susceptibility to TNF-alpha-induced apoptosis in blood cells and proteasome activity in aged human brain. In the present study, an in silico ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.056
更新日期:2006-04-01 00:00:00
abstract::In this work we demonstrate that heat and pressure induce only slightly different energetic changes in the unfolded state of RNase A. Using pressure and temperature as denaturants on a significant number of variants, and by determining the free energy of unfolding at different temperatures, we estimated the stability ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.038
更新日期:2006-03-01 00:00:00
abstract::Most excitatory synaptic transmissions in the central nervous system are mediated by the neurotransmitter glutamate. Binding of glutamate released from the presynaptic membrane causes glutamate receptors in the postsynaptic membrane to open, which results in a transient depolarization of the postsynaptic membrane. The...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.024
更新日期:2006-02-01 00:00:00
abstract::The kinin B2 receptor, which is constitutively expressed in a large number of tissues, mediates most of the known effects of bradykinin (BK). Normally undetectable in healthy tissues, the B1 receptor is strongly over-expressed under pathological conditions. BK is an important mediator in renal homeostasis and is mainl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.004
更新日期:2006-01-01 00:00:00
abstract::We developed a novel method for the simultaneous extraction and analysis of total tissue RNA and DNA to quantify the RNA and DNA oxidation products 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine using HPLC coupled to electrochemical detection (HPLC-ECD). The protein denaturing agents guanidine thio...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.014
更新日期:2006-01-01 00:00:00
abstract::Alternative splicing is a process by which multiple messenger RNAs (mRNAs) are generated from a single pre-mRNA, resulting in functionally distinct protein products. This is accomplished by the differential recognition of splice sites in the pre-mRNA, often regulated in a tissue- or development-specific manner. Altern...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2005.143
更新日期:2005-12-01 00:00:00
abstract::An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some branching via 1,4,6-linked glucose. We show that this polysaccharide scaffold is a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.131
更新日期:2005-11-01 00:00:00
abstract::During the last decade, an increasing number of new enzymes containing glycyl radicals in their active sites have been identified and biochemically characterised. These include benzylsuccinate synthase (Bss), 4-hydroxyphenylacetate decarboxylase (Hpd) and the coenzyme B12-independent glycerol dehydratase (Gdh). These ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2005.114
更新日期:2005-10-01 00:00:00
abstract::Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that is believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections is available to date, we and others have recently started to develop RNA interference (RNAi) approaches to prevent virus propagation. Here we d...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.100
更新日期:2005-09-01 00:00:00
abstract::Recombinant expression of actin in bacteria results in non-native species that aggregate into inclusion bodies. Actin is a folding substrate of TRiC, the chaperonin of the eukaryotic cytosol. By employing bacterial in vitro translation lysates supplemented with purified chaperones, we have found that TRiC is the only ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.088
更新日期:2005-08-01 00:00:00